Aptamer-Based Fluorometric Ochratoxin A Assay Based on Photoinduced Electron Transfer

A novel label-free cocaine assay based on aptamer-wrapped single-walled carbon nanotubes

Objective(s): This paper describes a selective and sensitive biosensor based on the dissolution and aggregation of aptamer wrapped single-walled carbon nanotubes. We report on the direct detection of aptamer–cocaine interactions, namely between a DNA aptamer and cocaine molecules based on near-infrared absorption at λ807. Materials and Methods: First a DNA aptamer recognizing cocaine was non-co...

Photoinduced Electron Transfer Based Ion Sensing within an Optical Fiber

We combine suspended-core microstructured optical fibers with the photoinduced electron transfer (PET) effect to demonstrate a new type of fluorescent optical fiber-dip sensing platform for small volume ion detection. A sensor design based on a simple model PET-fluoroionophore system and small core microstructured optical fiber capable of detecting sodium ions is demonstrated. The performance o...

Rotaxane-based molecular machines operated by photoinduced electron transfer

A molecular machine is an assembly of a definite number of molecular components designed to perform mechanical motions as a result of an appropriate external stimulation. Like their macroscopic counterparts, nanoscale machines need energy to operate. Energy can be supplied through (i) chemical reactions, (ii) electrochemical processes, and (iii) photoinduced processes. Although most molecular m...

Aptamer-based thrombin assay on microfluidic platform.

A facile and sensitive aptamer-based protocol has been developed for protein assay on microfluidic platform with fluorescence detection using an off-chip microarray scanner. Aptamer-functionalized magnetic beads were used to capture thrombin that binds to a second aptamer fluorescently labeled by Cy3. Experimental conditions, such as incubation time and temperature, washing time, interfering pr...

Nonequilibrium photoinduced electron transfer